高次生体モデル開発研究部　
主任研究員

根来　亮介

研究テーマ
高次 腸・肝・腎 in vitro モデルの開発

学位・資格

2015年

摂南大学薬学部卒業　薬剤師

2019年

大阪大学大学院薬学研究科　修了 博士（薬学）

所属学会

日本薬学会

日本薬物動態学会

日本組織培養学会

MPS実用化推進協議会

主要論文

Ryosuke Negoro*, Sayaka Deguchi, Daiju Yamazaki, Kazuo Takayama, Takuya Fujita Genome edited intestine liver on a chip system for integrated intestinal hepatic drug absorption and metabolism evaluation. Sci. Rep., 15, 38609 (2025). *Corresponding author.

Ryosuke Negoro*, Ayu Ouchi, Sayaka Deguchi, Kazuo Takayama, Takuya Fujita. Refining Hepatocyte Models to Capture the Impact of CYP2D6*10 Utilizing a PITCh System. Biol. Pharm. Bull., 47(8), b24-00202 (2024). *Corresponding author.

Keita Watanabe#, Ryosuke Negoro#*, Takuya Fujita. 5-ALA treatment increases intracellular heme levels and enhances CYP3A4 activity in genome-edited Caco-2 cells. Biochem. Biophys. Res. Commun., 664, 94–99 (2023). # These authors contributed equally to this work. *Corresponding author.

Naoki Yamada#, Ryosuke Negoro#*, Keita Watanabe, Taluya Fujita. Generation of Caco-2 cells with predictable metabolism by CYP3A4, UGT1A1 and CES using the PITCh system. Drug Metab. Pharmacokinet. 100497 (2023). # These authors contributed equally to this work. *Corresponding author.

Ryosuke Negoro#*, Mitsuki Tasaka#, Sayaka Deguchi, Kazuo Takayama, Takuya Fujita. Generation of HepG2 Cells with High Expression of Multiple Drug-Metabolizing Enzymes for Drug Discovery Research Using a PITCh System. Cells, 11, 1677 (2022). # These authors contributed equally to this work. *Corresponding author.

Ryosuke Negoro#*, Naoki Yamada#, Keita Watanabe, Yusuke Kono, Takuya Fujita. Generation of Caco-2 cells stably expressing CYP3A4·POR·UGT1A1 and CYP3A4·POR·UGT1A1*6 using a PITCh system. Arch. Toxicol, 96, 499–510 (2022). # These authors contributed equally to this work. *Corresponding author.

Moe Ichikawa#, Ryosuke Negoro#, Kanae Kawai, Tomoki Yamashita, Kazuo Takayama, Hiroyuki Mizuguchi. Vinblastine treatment enhances the intestinal functions and decreases the undifferentiated cell contamination of human iPS cell-derived intestinal epithelial-like cells. Mol. Ther. Methods. Clin. Dev., 20, 463–472 (2021). # These authors contributed equally to this work.

Ryosuke Negoro#, Kanae Kawai#, Moe Ichikawa, Sayaka Deguchi, Kazuo Takayama, Hiroyuki Mizuguchi. Establishment of MDR1-knockout human induced pluripotent stem cell line. Drug Metab. Pharmacokinet., 35, 288–296 (2020). # These authors contributed equally to this work.

Kanae Kawai#, Ryosuke Negoro#, Moe Ichikawa, Tomoki Yamashita, Sayaka Deguchi, Kazuo Harada, Kazumasa Hirata, Kazuo Takayama, Hiroyuki Mizuguchi. Establishment of SLC15A1/PEPT1-knockout human induced pluripotent stem cell line for intestinal drug absorption studies. Mol. Ther. Methods. Clin. Dev., 17, 49-57 (2020). # These authors contributed equally to this work.

Kazuo Takayama#, Ryosuke Negoro#, Tomoki Yamashita, Kanae Kawai, Moe Ichikawa, Takanori Mori, Noriyuki Nakatsu, Kazuo Harada, Sumito Ito, Hiroshi Yamada, Yoshiyuki Yamaura, Kazumasa Hirata, Seiichi Ishida, Hiroyuki Mizuguchi. Generation of Human iPSC–Derived Intestinal Epithelial Cell Monolayers by CDX2 Transduction. Cell. Mol. Gastroenterol. Hepatol., 8, 513–526 (2019). # These authors contributed equally to this work.